{"id":8330,"date":"2023-06-07T17:31:23","date_gmt":"2023-06-07T17:31:23","guid":{"rendered":"https:\/\/enacif.unam.mx\/?p=8330"},"modified":"2023-06-07T17:36:27","modified_gmt":"2023-06-07T17:36:27","slug":"articulo-de-la-enacif-a-mirnome-analysis-at-the-early-postmortem-intervalarticulo-de-la-enacif","status":"publish","type":"post","link":"https:\/\/enacif.unam.mx\/?p=8330","title":{"rendered":"Art\u00edculo de la ENaCiF: A miRNome analysis at the early postmortem interval"},"content":{"rendered":"\n[et_pb_section fb_built=\u00bb1″ theme_builder_area=\u00bbpost_content\u00bb _builder_version=\u00bb4.19.2″ _module_preset=\u00bbdefault\u00bb][et_pb_row _builder_version=\u00bb4.19.2″ _module_preset=\u00bbdefault\u00bb theme_builder_area=\u00bbpost_content\u00bb][et_pb_column _builder_version=\u00bb4.19.2″ _module_preset=\u00bbdefault\u00bb type=\u00bb4_4″ theme_builder_area=\u00bbpost_content\u00bb][et_pb_text _builder_version=\u00bb4.19.2″ _module_preset=\u00bbdefault\u00bb theme_builder_area=\u00bbpost_content\u00bb hover_enabled=\u00bb0″ sticky_enabled=\u00bb0″]

Disponible v\u00eda: https:\/\/peerj.com\/articles\/15409\/<\/a><\/p>\n

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Abstract<\/h2>\n
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The postmortem interval (PMI) is the time elapsing since the death of an individual until the body is examined. Different molecules have been analyzed to better estimate the PMI with variable results. The miRNAs draw attention in the forensic field to estimate the PMI as they can better support degradation. In the present work, we analyzed the miRNome at early PMI in rats\u2019 skeletal muscle using the Affymetrix GeneChip\u2122 miRNA 4.0 microarrays. We found 156 dysregulated miRNAs in rats\u2019 skeletal muscle at 24 h of PMI, out of which 84 were downregulated, and 72 upregulated. The miRNA most significantly downregulated was miR-139-5p (FC = \u2212160,\u00a0<\/span>p<\/i>\u00a0<\/span>= 9.97 \u00d7 10\u221211<\/sup>), while the most upregulated was rno-miR-92b-5p (FC = 241.18,\u00a0<\/span>p<\/i>\u00a0<\/span>= 2.39 \u00d7 10\u22126<\/sup>). Regarding the targets of these dysregulated miRNAs, the rno-miR-125b-5p and rno-miR-138-5p were the miRNAs with more mRNA targets. The mRNA targets that we found in the present study participate in several biological processes such as interleukin secretion regulation, translation regulation, cell growth, or low oxygen response. In addition, we found a downregulation of\u00a0<\/span>SIRT1<\/i>\u00a0<\/span>mRNA and an upregulation of\u00a0<\/span>TGFBR2<\/i>\u00a0<\/span>mRNA at 24 h of PMI. These results suggest there is an active participation of miRNAs at early PMI which could be further explored to identify potential biomarkers for PMI estimation.<\/p>\n<\/div>\n<\/div>\n<\/div>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Disponible v\u00eda: https:\/\/peerj.com\/articles\/15409\/ Abstract \u00a0 The postmortem interval (PMI) is the time elapsing since the death of an individual until the body is examined. Different molecules have been analyzed to better estimate the PMI with variable results. The miRNAs draw attention in the forensic field to estimate the PMI as they can better support degradation. […]<\/p>\n","protected":false},"author":2,"featured_media":8331,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[29,33,24,3,13],"tags":[],"class_list":["post-8330","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articulo","category-articulobio","category-cabecera","category-eventos","category-paper"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/posts\/8330"}],"collection":[{"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8330"}],"version-history":[{"count":5,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/posts\/8330\/revisions"}],"predecessor-version":[{"id":8337,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/posts\/8330\/revisions\/8337"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=\/wp\/v2\/media\/8331"}],"wp:attachment":[{"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8330"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8330"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/enacif.unam.mx\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8330"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}